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. 2002 May 15;109(10):1335–1344. doi: 10.1172/JCI14989

Figure 6.

Figure 6

The activation and proliferation of allogeneic CD8+ T cells depend on the presence of host DCs and require MHC class I expression on host cells. (a) CFSE-labeled CD8+ T cells (2 × 106) from C3H.SW mice (CD45.2+) were transplanted into irradiated B6 recipients (CD45.1+) at the indicated timepoints after TBI. Five days later, splenocytes were prepared from these mice for flow cytometry analysis as described in Figure 5b legend. (b) C3H.SW CFSE-CD8+CD45.2+ T cells (2 × 106) were intravenously transferred into lethally irradiated β2m–/– mice or congenic B6/SJL (CD45.1+) recipients. Splenocytes were prepared from these mice, and the division of donor CD8+ T cells was analyzed by flow cytometry. The absolute number (c) of C3H.SW CD8+ T cells in the spleens of recipients 5 days after transplantation was calculated based on the flow cytometry analysis. Data shown derive from five independent experiments. One representative experiment of three is shown.