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. 2002 May 15;109(10):1291–1302. doi: 10.1172/JCI15182

Figure 2.

Figure 2

Phenotypic characterization of hMAPC differentiation to hepatocyte-like cells. The hMAPCs were cultured on Matrigel at 3 × 104 cells/cm2 with FGF-4 for 21 days. Representative example of three experiments. Scale bar, 25 μm. (a and d) Day 0 and 14, stained with albumin-FITC and HNF-1α-Cy5 Ab’s. (b and e) Day 7 and 14, stained with CK18-FITC and GATA4-Cy5 Ab’s. (c and f) Day 7 and 14, stained with CK8-FITC and HNF-3β-Cy5 Ab’s. (g–i) Day 21, stained with CK18-Cy3 and albumin-FITC Ab’s. (g) CK18-Cy3, (h) albumin-FITC, and (i) overlay. (j–l) Day 21, stained with CK8-Cy3 and CK18-FITC Ab’s. (j) CK8-Cy3, (k) CK18-FITC, and (l) overlay.