Figure 3.

Single hMAPC (clone A16) that differentiates to mesoderm and neuroectoderm, differentiates to hepatocyte-like cells when cultured with FGF-4 on Matrigel for 21 days. The hMAPCs were from clone A16, described elsewhere (M. Reyes, unpublished observations), generated from cultures in which five eGFP⁺ hMAPCs were added to 95 nontransduced hMAPCs and expanded to more than 5 × 10⁶ cells. A16-derived hMAPCs were cultured with FGF-4 on Matrigel for 14 days and stained with Ab’s against CK18, albumin, and HNF-3β. Slides were evaluated by confocal microscopy for coexpression of eGFP and hepatocyte markers. Scale bar, 25 μm. (a–c) FGF-4-induced MAPC evaluated for eGFP expression. (d–e) Same field as a and b stained with CK18-Cy3. eGFP⁺ cells express CK18. (f) Same field as c stained for albumin-Cy3 and HNF-3β–Cy5. eGFP⁺ cells express albumin and HNF-3β.