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. 2002 May 15;109(10):1327–1333. doi: 10.1172/JCI15417

Figure 3.

Figure 3

Immunoblots and assays for DNA-binding of MEF2 using WV muscle extracts from mice with wild-type (+/+), heterozygous (+/–), and homozygous (–/–) mutant CLCN1 alleles. (a) Western blot analyses of β-galactosidase, major MEF2 isoforms (MEF2A, -2C, and -2D), myoglobin, active form of p38 MAPK (phospho-p38 MAPK), and total p38 MAPK. Tubulin blot showed equivalent loading of each lane. (b) Electrophoretic mobility shift assay of proteins extracted from WV muscles of different mice. The identity of the MEF2 protein-DNA complex (arrow) was confirmed by competition with 100-fold molar excess of unlabeled MEF2-binding oligonucleotides (competitor).