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. 1999 Jan 5;96(1):97–102. doi: 10.1073/pnas.96.1.97

Figure 2.

Figure 2

GLC analyses. (A) Authentic 24Z- and 24E-ethylidenelanost-8-en-3β-ol mixture analyzed by using GLC with flame ionization detection. The major component (24Z isomer) comprised 85% and the minor component (24E isomer) comprised 15% of the chemically synthesized product (a). Co-chromatography of the authentic standard mix and GLC-purified P. carinii hominis pneumocysterol. The minor 24E-isomer peak was unchanged, whereas the major 24Z-isomer peak was enhanced (b). (B) Effects of formalin fixation on rat PcP-lung free sterols. Formalin-fixed rat PcP lungs stored for 12 weeks at room temperature (a); freshly isolated rat PcP lungs (b); and purified P. carinii carinii organisms (c). (C) Pneumocystis carinii hominis sterols. Total sterols from a formalin-fixed PcP lung (a); total sterols from organisms isolated from a frozen PcP lung (b); total sterols of BALF from a PcP patient (c); free sterol fraction from a formalin-fixed PcP lung (d); and steryl ester sterols from a formalin-fixed PcP lung (e). The ratios of peak 24 (pneumocysterol) to peak 13 (fungisterol) were: a, 21.9; b, 2.5; c, 2.2; d, 21.8; and e, 0.3.