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. 2002 Jun 15;109(12):1635–1643. doi: 10.1172/JCI15402

Figure 1.

Figure 1

Inhibition of binding of biotinylated MBP 86-100 to HLA-DR2 molecules by different competitors. Recombinant water-soluble HLA-DR2 molecules were incubated with biotinylated MBP 86-100 (0.13 μM) and the unlabeled random copolymers at a range of concentrations. All incubations were carried out in duplicate at pH 7.0 for 40 hours at 37°C. Results represent one of three independent experiments. Specific binding is expressed as percentage of inhibition using the formula: percentage of inhibition = 100% – [(absorbance at 410 nm with competitor – background)/absorbance without competitor – background) × 100]. The signals at 410 nm without competitor were 0.8–1.0, and the background was 0.1.