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. 2002 Jun 15;109(12):1541–1550. doi: 10.1172/JCI15420

Figure 5.

Figure 5

Overexpression of WT Rab7 or Rab9 (but not Rab11) in NP-C cells reduced the accumulation of intracellular cholesterol. (a) Cells were transfected with plasmids expressing EGFP fusion proteins of WT Rab7, Rab9, or Rab11 and cultured in EMEM plus 10% FBS. Forty-eight hours later, the distribution of free cholesterol was examined by filipin staining (blue fluorescence). Transfected cells identified by green fluorescence are outlined in the filipin images. Bar, 25 μm. (b) Quantitation of filipin fluorescence in NP-C cells overexpressing WT Rab7, Rab9, or Rab11 proteins. Total filipin fluorescence of individual transfected or adjacent untransfected cells was calculated by image analysis. Values shown are mean ± SD from a typical experiment (n = 50). Similar results were obtained in each of three independent experiments.