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. 2002 Jun 15;109(12):1541–1550. doi: 10.1172/JCI15420

Figure 6.

Figure 6

Overexpression of WT Rab7 or Rab9 (but not Rab11) in NP-C cells increased the accumulation of neutral lipids. (a) Cells were transfected with plasmids expressing EGFP fusion proteins of WT Rab7, Rab9, or Rab11 as in Figure 5. Forty-eight hours later, samples were fixed and stained for the presence of cholesterol esters and other neutral lipids using Nile Red (34). Transfected cells identified by green fluorescence are outlined on the Nile Red images. Bar, 25 μm. (b) Quantitation of Nile Red fluorescence in NP-C cells overexpressing WT Rab7, Rab9, or Rab11 proteins. Total Nile Red fluorescence of individual transfected or adjacent untransfected cells was calculated by image analysis. Values are mean ± SD from a typical experiment (n = 30). Similar results were obtained in each of three independent experiments.