Figure 2.

Inhibition of selectin-Ig binding to immobilized PAA-SLe^X. Inhibition curves were generated using OS-heparin (open circles), heparin (open squares), NDS-heparin (asterisks), 2/3DS-heparin (open triangles), N/2/3DS-heparin (filled triangles), 6DS-heparin (filled squares), and CR-heparin (filled circles) (see Methods). Inclusion of a mAb to P-selectin, E-selectin, or EDTA blocked binding by more than 90%. Each point represents the average of duplicate determinations, and the data are representative of three or four separate experiments.