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Transduction of CHO cells with BacMam1 GFP baculovirus. (A and B) Visualization of GFP-expressing cells by fluorescence microscopy. (A) Cells transduced with BacMam1 GFP. (B) Same as A except for addition of 1 mM butyrate after incubation with virus. (C) Analysis of GFP expression by flow cytometry. Black line, nontransduced cells; blue line, transduced cells; red line, transduced cells plus 1 mM butyrate; green line, same as red line but with neutral-density filter in place.
