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Western blot analysis of GFP and NPT II expression in CHO cells. Extracts of cells were prepared 24 hr posttransduction. Lanes 1–3, cells probed with antibody directed against GFP; lanes 4–6, probed with antibody directed against NPT II. Lanes 1 and 4, nontransduced cells; lanes 2 and 5, transduced cells; lanes 3 and 6, transduced cells plus 1 mM butyrate. KDa, kilodaltons.
