Table 1.
Transduction of mammalian cell lines with BacMam1 GFP baculovirus
| Cells | Percent transduced
|
Mean fluorescence, MESF × 10−6
|
||
|---|---|---|---|---|
| Virus | Virus + 10 mM butyrate | Virus | Virus + 10 mM butyrate | |
| HuH-7 | 91 ± 1.3 | 100 ± 0.2 | 1.2 ± 0.1 | 24.4 ± 1.5 |
| CHO | 72 ± 1.8 | 97 ± 0.7 | 1.1 ± 0.1 | 7.6 ± 0.6 |
| CHO-T Ag | 94 ± 2.5 | 99 ± 0.2 | 3.4 ± 0.3 | 16.2 ± 0.8 |
| CV-1 | 89 ± 1.7 | 95 ± 0.7 | 8.0 ± 0.4 | 46.8 ± 1.8 |
| CosV-7 | 86 ± 1.5 | 94 ± 1.2 | 8.9 ± 0.5 | 62.5 ± 4.1 |
| HeLa | 83 ± 2.6 | 98 ± 0.5 | 2.5 ± 0.3 | 56.6 ± 2.0 |
| 293 | 85 ± 2.8 | 98 ± 0.4 | 3.4 ± 0.3 | 15.9 ± 0.3 |
| BHK | 91 ± 1.2 | 99 ± 0.5 | 2.1 ± 0.06 | 52.9 ± 1.2 |
| WI38 | 84 ± 6.1 | 99 ± 0.3 | 12.3 ± 0.2 | 71.5 ± 4.1 |
| SK-N-MC | 90 ± 0.1 | 100 ± 0.1 | 1.4 ± 0.07 | 31.4 ± 1.2 |
| PC12 | 1 ± 0.3 | 19 ± 0.5 | 0.004 ± 0.003 | 0.34 ± 0.03 |
| Human keratinocytes | 61 ± 2.4 | 84 ± 1.7 | 1.7 ± 0.04 | 9.4 ± 0.8 |
| W12 | 89 ± 0.7 | 98 ± 0.5 | 19.2 ± 0.4 | 47.8 ± 0.7 |
| HBMF | 46 ± 2.9 | 84 ± 2.1 | 0.44 ± 0.04 | 6.6 ± 0.7 |
| MG63 | 92 ± 2.9 | 97 ± 0.7 | 2.6 ± 0.5 | 17.1 ± 3.4 |
| THP-1 (+PMA) | 7 ± 0.7 | 5 ± 0.8 | 0.007 ± 0.003 | 0.04 ± 0.008 |
| K562 | 1 ± 0.1 | 18 ± 1.2 | 0.007 ± 0.005 | 0.12 ± 0.004 |
| Raw264.7 | 2 ± 0.3 | 8 ± 0.5 | 0.007 ± 0.001 | 0.03 ± 0.008 |
| P388D1 | 2 ± 0.2 | 26 ± 5.3 | 0.002 ± 0.0004 | 0.4 ± 0.12 |
| U937 | 0 | 0 | 0 | 0 |
Results are averages of three independent transductions. Errors are standard deviations. MESF, molecules of equivalent soluble fluorochrome. PMA, phorbol 12-myristate 13-acetate.