Fig. 5. RNA analysis of the different pre-40S particles. (A) Primer extension to detect 35S, 20Smeth., 18S and 27SA2 rRNA, and (B) northern hybridization to detect 7S rRNA and U3 snoRNA were performed with RNA extracted from affinity-purified TAP-tagged Enp1p, Dim1p, Rrp12p, Hrr25p, Tsr1p, Rio2p, Nob1p, Nsa3p, and a non-tagged wild-type strain (negative control). Oligonucleotides used are indicated to the left of each corresponding panel, and their annealing position in the pre-rRNAs is depicted in (C).