Table I. Identification of Enp1p-associated proteins from TAP purifications of whole-cell lysate (WCL), 90S ribosomal precursors (90S pool) and pre-40S particles (40S pool, see Figure 1B).
| Mol. wt (kDa) | WCE | 90S pool | Band | 40S pool | |||
|---|---|---|---|---|---|---|---|
| 287.4 | Utp20p | YBL004W | Utp20p | YBL004W | 1 | ||
| 193.0 | Rrp5p | YMR229C | Rrp5p | YMR229C | 2 | ||
| 199.9 | Utp10p | YJL109C | Utp10p | YJL109C | 3 | ||
| 144.8 | Ecm16p | YMR128W | Ecm16p | YMR128W | 4 | ||
| 140.4 | Utp22p | YGR090W | Utp22p | YGR090W | 5 | ||
| 135.6 | Bms1p | YPL217C | Bms1p | YPL217C | 5 | ||
| 137.5 | Rrp12p | YPL012W | Rrp12p | YPL012W | Rrp12p | YPL012W | |
| 119.2 | Kre33p | YNL132W | Kre33p | YNL132W | 6 | ||
| 106.2 | Dip2p | YLR129W | Dip2p | YLR129W | 7 | ||
| 103.9 | Pwp2p | YCR057C | Pwp2p | YCR057C | 7 | ||
| 104.7 | Utp21p | YLR409C | Utp21p | YLR409C | 8 | ||
| 94.2 | Nop14p | YDL148C | Nop14p | YDL148C | 8 | Nop14p | YDL148C |
| 90.6 | Tsr1p | YDL060W | Tsr1p | YDL060W | |||
| 101.1 | Nan1p | YPL126W | 9 | ||||
| 91.0 | Utp13p | YLR222C | Utp13p | YLR222C | 10 | ||
| 81.6 | Enp2p | YGR145W | 11 | ||||
| 87.8 | Utp4p | YDR324C | Utp4p | YDR324C | 11 | ||
| 66.8 | Mpp10p | YJR002W | Mpp10p | YJR002W | 12 | ||
| 80.0 | Utp8p | YGR128C | Utp8p | YGR128C | 13 | ||
| 61.2 | Bfr2p | YDR299W | 14 | ||||
| 72.0 | Utp5p | YDR398W | Utp5p | YDR398W | 14 | ||
| 64.9 | Rrp9p | YPR137W | 15 | ||||
| 65.4 | Ded1p | YOR204W | 16 | ||||
| 65.1 | Utp9p | YHR196W | Utp9p | YHR196w | 17 | ||
| 63.7 | Rok1p | YGL171W | 18 | ||||
| 62.2 | Utp7p | YER082C | Utp7p | YER082C | 18 | ||
| 55.0 | Enp1p | YBR247C | Enp1p | YBR247C | Enp1p | YBR247C | |
| 53.2 | Ltv1p | YKL143W | 19 | Ltv1p | YKL143W | ||
| 56.8 | Nop58p | YOR310C | Nop58p | YOR310C | 20 | ||
| 63.5 | Utp19p | YPR144C | Utp19p | YPR144C | 21 | ||
| 56.7 | Sik1p | YLR197W | Sik1p | YLR197W | 21 | ||
| 57.4 | Hrr25p | YPL204W | Hrr25p | YPL204W | |||
| 51.6 | Nob1p | YOR056C | Nob1p | YOR056C | |||
| 56.8 | Sof1p | YLL011W | 22 | ||||
| 57.6 | Utp15p | YMR093W | 23 | ||||
| 49.1 | Rio2p | YNL207W | Rio2p | YNL207W | |||
| 52.3 | Utp6p | YDR449C | Utp6p | YDR449C | 24 | ||
| 33.5 | Imp4p | YNL075W | Imp4p | YNL075W | 25 | ||
| 35.8 | Dim1p | YPL266W | Dim1p | YPL266W | |||
| 34.7 | Asc1p | YMR116C | Asc1p | YMR166C | 26 | Asc1p | YMR166C |
| 30.2 | YOR145C | YOR145C | YOR145C | ||||
| 30.6 | Bud23p | YCR047C | 27 | Bud23p | YCR047C | ||
| 31.5 | YKR060W | 28 | |||||
| 30.2 | Utp11p | YKL099C | 29 |
Ribosomal proteins S0, S1, S2, S5, S6, S7, S8, S9, S11, S13, S14, S16, S18, S19, S22, S23, S24, S25, L3, L16 and L17 were also found in the WCL. Co-purifying proteins were identified by mass spectrometry; both ORF and SGD standard names are listed. Bands 1–29 correspond to identified proteins in the 90S pool. Major co-purifying proteins of the 40S pool are in bold. Contaminants (e.g. heat shock proteins) are not depicted.