Figure 5.

Activation of a 34-kDa kinase by alendronate and lovastatin is blocked by geranylgeraniol and, to a lesser extent, mevalonate. Ocls were purified from cocultures (see Fig. 1) by sequential treatment of culture dishes with collagenase and then EDTA, as described in Materials and Methods. Ocls then were left untreated (lane 1) or were incubated with ALN (30 μM; lanes 2–5) or LOV (10 μM; lanes 6–9) in the presence of MVA (1 mM; lanes 3 and 7), FOH (10 μM; lanes 4 and 8), or GGOH (10 μM; lanes 5 and 9) for 17 hr. Cell lysates were analyzed by in-gel kinase assay using myelin basic protein as a substrate. Activity of 34-kDa (arrow) and 36-kDa kinases was visualized by autoradiography. Data is representative of three independent experiments.