Fig. 3. Cellular localization of RBM4 and a CAD fusion protein. (A) Untransfected HeLa cells were stained with anti-RBM4 antibodies (Endogenous), whereas cells transfected with the expression vector of HA-tagged RBM4 were subjected to staining with anti-HA antibody (Transient expression). The arrow indicates an even nucleoplasmic staining pattern of RBM4.HA, whereas the triangle marks the cell where RBM4.HA is concentrated in nucleoli. Cells visualized by phase-contrast microscopy are also shown. (B) HeLa cells were transfected with the vector expressing GFP–CAD, and immunostaining with anti-SC35 antibody was performed. Merge: images of fluorescent fusion protein and immunostaining are merged.