Fig. 6. Uptake of DNA is not related to MPT. (A) DNA uptake is inhibited by CaCl₂. Mitochondrial incorporation of labeled maize 2.3 kb plasmid was tested in the presence of increasing concentrations of CaCl₂. (B) DNA uptake is inhibited in oxidative conditions. Incorporation of labeled maize 2.3 kb plasmid was tested with mock-treated mitochondria (Cont) and with mitochondria pretreated with H₂O₂ and FeSO₄ (Fenton’s reagent, Fent). (C) DNA uptake is inhibited by thiol oxidation. Mitochondrial incorporation of labeled maize 2.3 kb plasmid was tested in the absence (Cont) and presence (Dia) of 3 mM diamide. Migration of the incorporated plasmid (2.3PL) is indicated.