Figure 5.

Examples of flow cytometry data obtained with a Graves hyperthyroid serum and serum from a hypothyroid patient with TBAb activity. Note that despite giving an excellent fluorescent signal on the ECD-GPI cell line, the same Graves serum does not recognize the TSHR-10,000 cells overexpressing the TSH holoreceptor on their surface (a). In contrast, the serum from a hypothyroid patient with TBAb activity recognizes both ECD-GPI and TSHR-10,000 cells equally well (b). Serum from a normal individual is included as a control (c).