Fig. 2. SIMK and MMK3 are activated specifically by ethylene production. Effect of α-aminoisobutyric acid (AIB), an inactive analog of ACC, or of the inhibition of ACC oxidase by amino-oxyacetic acid (AOA). After treatment with AIB (1 mM) or AOA (0.5 mM), SIMK and MMK3 activities were determinated at different times by immunocomplex kinase assay using 5 µg of protein A-purified M23- and M14-specific antibodies.