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. 1999 Jan 5;96(1):145–150. doi: 10.1073/pnas.96.1.145

Figure 2.

Figure 2

Overexpression of CED-4 induces cell death and caspase activation in Drosophila S2 cells. (A) Overexpression of CED-4 kills Drosophila S2 cells. Cells were transfected with ced-4. At 24 h after heat-shock treatment, cells were fixed and stained with Hoechst 33342. Open arrowhead indicates condensed nuclei. Scale Bar, 10 μm. (B) Quantification of cell death in Drosophila S2 cells. The data (mean ± SD) are the percentage of cells with condensed chromatin in the total number of cells counted. (C and D) DEVD- and YVAD- cleaving activities of the cytoplasmic lysates of S2 cells expressing various proteins or treated with 1 μM staurosporine. S2 cells were transfected with various plasmids, and lysates were prepared 24 h after heat-shock treatment.