Fig. 3. Distribution of sperm crossovers across the Eβ hotspot. (A) Cumulative frequency distributions of sperm crossovers in the four hybrids analysed, for the combined orientation A + B recombinants. The least-squares best-fit cumulative frequency distribution assumes that crossovers are normally distributed across the hotspot (centre at 7260 bp, standard deviation 350 bp). (B) Cumulative frequency distributions of separate orientation A (filled squares) and B (open squares) crossovers. The best-fit line for the combined A + B distributions is indicated. The B distributions are shifted 3′ relative to A distributions in all crosses.