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. 1999 Jan 5;96(1):151–156. doi: 10.1073/pnas.96.1.151

Figure 1.

Figure 1

In vivo BRET in E. coli cells expressing the RLUC–EYFP fusion protein. (A) Diagrams of the expression cassettes of pT7/Eyfp, pT7/Rluc, and pT7/Rluc•Eyfp. PT7, T7 promoter region; Ter, transcription terminator region. The intergenic linker sequence of 11 amino acid residues between Rluc and Eyfp in the Rluc•Eyfp fusion construct is shown below the pT7/Rluc•Eyfp diagram. (B) Luminescence (lum.) or fluorescence (fluor.) emission spectra from transformed strains expressing the proteins RLUC, EYFP, or the fusion protein RLUC•EYFP. Luminescence reactions were initiated by the addition of 1 μM coelenterazine. All spectra were normalized.