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KaiB–KaiB association in vitro revealed by BRET. Extracts containing fusion proteins EYFP•KaiB were mixed in equal proportions with those containing RLUC•KaiB. Bioluminescence emission profiles were measured at the indicated times after the extracts were combined and incubated at room temperature. Fresh coelenterazine (1 μM) was added at the beginning of the reaction at each time point.
