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Fig. 5. MSK1 phosphorylates p65 in vivo. (A) HEK293 cells, transiently transfected with the relevant expression vectors, were labeled with [32P]orthophosphate for 4 h. Cell lysates were subjected to immunoprecipitation with anti-p65 antibody. Precipitated proteins were separated by SDS–PAGE and analyzed using PhosphorImager technology. (B) Similar experiment to that in (A), but immunoprecipitation was performed with anti-Flag antibody.
