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Construction and expression of MUC1-specific single-chain T cell receptors (scTRs). (a) 293H cells were transfected with (b) the scTR, (c) scTR-CD4TM-hZ, or (d) scTR-CD4TM-AGD-hZ mammalian expression vectors. Cells were stained with anti-TR BF1 (open histogram) or isotype control (filled histogram) antibody. (e) shows quantitative comparison of TR expression on 293H cells transfected with different scTR constructs. P < .05.
