Figure 4.

Vitamin E blocks binding of NF-κB and AP-1 to the CD95L promoter target sites. (a) ³²P-labeled oligonucleotides containing the CD95L NF-κB (at the promoter –530 and –50 region) and AP-1 (at the promoter +90 region) binding sequences were analyzed by EMSA. Nuclear extracts were prepared from J-27 T cells either not induced (–) or induced (+) by PMA and ionomycin in the absence (–) or presence (+) of vitamin E. The inducible complexes corresponding to NF-κB and AP-1 are indicated. An NF-Y binding oligonucleotide was used for equal loading of nuclear extracts. (b) EMSA was carried out in the absence (–) or presence (+) of the antibodies indicated.