Figure 5.

Vitamin E downregulates the CD95L promoter and CD95L NF-κB– or CD95L AP-1–mediated transcriptional activity. J-27 T cells were transiently transfected with the luciferase reporter constructs containing the 0.86-kb CD95L promoter, or three copies of the NF-κB binding sites at the –530 or –50 region or of the AP-1 motif at the +90 region of the CD95L promoter. A luciferase reporter construct containing three copies of the Egr consensus binding site was used as a control. After overnight culture, the cells were divided and treated with vitamin E or the vitamin E solvent ethanol for 1 hour and then further cultured in the absence (white bars) or presence (black bars) of PMA and ionomycin. Luciferase activity was determined after 8 hours of stimulation. Results represent the average of three to five independent transfection experiments.