Figure 3.

Levels of plasma sterols and lipoprotein profiles of ABCG5 and ABCG8 transgenic mice. Male and female 12-week-old transgenic mice and their littermate controls (n = 4–7 in each group) were maintained on a chow diet. After a 4-hour fast, the animals were sacrificed and the venous blood collected. The plasma was isolated by centrifugation, the plasma levels of cholesterol were measured as described in Methods, and the noncholesterol sterol levels of plasma were assayed by gas chromatography as described (8). Pooled plasma from each group of mice was subject to fast protein liquid chromatography fractionation, and the cholesterol content in each fraction was assayed as described in Methods. WT, wild-type; Tg, transgenic. *P < 0.05, **P < 0.01.