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Biochemistry of ligation reaction. (Left) pH dependence and (Center and Right) effect of replacing Mg2+ with a series of divalent cations using ribozyme/substrate complex in Fig. 1c. (Center) kobs as a function of Mg2+ (⋄) and Mn2+ (□) at pH 7.0. (Right) kobs as a function of Mg2+ (□), Ca2+ (⋄), Cd2+ (○), Sr2+ (▵), and Ba2+ (crossed squares) at pH 9.0.
