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. 2002 Oct 1;110(7):933–941. doi: 10.1172/JCI14268

Figure 4.

Figure 4

Unligated integrin α5β1 induces caspase-3 and -8, but not -9, activation. HUVECs were plated on (a) fibronectin-, (b) vitronectin-, or (a and b) poly-L-lysine–coated culture plates in the presence of anti-α5β1 Ab’s and 50 μM z-DEVD-fmk (caspase-3) or z-IETD-fmk (caspase-8) inhibitors or vehicle control (0.33% DMSO) for 24 hours. The percentage of annexin V–positive cells was then determined. inhib, inhibitor. (c and d) Caspase-3 and -8 activities were determined in HUVECs plated on vitronectin-coated or poly-L-lysine–coated plates in the presence of culture medium, anti-α5β1, or control Ab’s. (e) Cell lysates were immunoblotted with anti–caspase-3 and anti–cleaved caspase-3 Ab’s. Relative caspase-3 cleavage was determined by densitometry. (f) Cell lysates were immunoblotted with anti–caspase-9 and anti–cleaved caspase-9 Ab’s.