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. 1999 Jan 5;96(1):191–196. doi: 10.1073/pnas.96.1.191

Figure 1.

Figure 1

Initiation of V(D)J recombination as revealed by the presence of signal ends at the nonpermissive temperature. (A) Diagrammatic representation of the Jκ-gene loci, along with the predicted sizes of signal ends detected by LM-PCR. Arrows indicate the primers used to amplify the excised signal ends. (B) LM-PCR analysis of signal ends. DNA samples isolated from the scid heterozygous (s/+-ts) and scid homozygous (s/s-ts) cells, cultured either at 33°C (L) or at 39°C (H) were subjected to an LM-PCR assay to assess the level of recombination signal ends from the Jκ gene loci. Amplification of the actin gene is used as a control for the amount of input DNA.