Expression of GST-C32 and BCR-ABL in 27–1 cells. GST-C32 was overexpressed alone or in combination with P210 or P185 BCR-ABL in 27–1 cells. Total cell lysates (a and b) or glutathione Sepharose-bound preparations (c–f) from cells expressing GST (vector alone) (lane 1), GST-C32 (lane 2), GST-C32/P185 BCR-ABL (lane 3), GST-C32/P210 BCR-ABL (lane 4), GST/P185 BCR-ABL (lane 5), and GST/P210 BCR-ABL (lane 6) were subjected to anti-ABL (a and c), anti-XPB (b and d), anti-p62 (e), and anti-phosphotyrosine (PY) (f) Western blotting.