Table 4.
Estimates of pairwise sequence conservation in coding and non-coding regions between D. melanogaster and D. erecta, D. pseudoobscura, D. willistoni or D. littoralis
| Species | Number of bp surveyed | Number of bp conserved | % conserved (bp) | Median length of conserved segment | Average % identity of conserved segment |
| Coding | |||||
| D. erecta | 63,655 | 60,327 | 94% | 39 | 93% |
| D. pseudoobscura | 46,626 | 26,978 | 61% | 20 | 91% |
| D. willistoni | 42,224 | 18,774 | 45% | 17 | 91% |
| D. littoralis | 19,717 | 10,997 | 63% | 17 | 92% |
| Non-coding | |||||
| D. erecta | 272,366 | 186,895 | 69% | 24 | 94% |
| D. pseudoobscura | 276,731 | 77,391 | 28% | 17 | 95% |
| D. willistoni | 174,421 | 19,700 | 13% | 14 | 95% |
| D. littoralis | 138,866 | 24,633 | 18% | 15 | 95% |
| D. virilis | 114,015 | 30,564 | 27% | 16 | 95% |
| D. virilis [44] | 114,015 | 29,915 | 26% | 19 | 93% |
Microsyntenic regions were globally aligned using AVID and conserved sequences greater than or equal to 10 bp and 90% identity were identified using VISTA. Sequences were classified as coding or non-coding using Release 3 annotations [14] exported from GadFly in VISTA format. Shown for comparison are a re-analysis of conservation between D. melanogaster and D. virilis using the current methods, as well as previous results, for a sample of non-coding regions published in [44].