Figure 2.

Expression Analysis of Transgenic Plants.

(A) to (D) PNH antisense RNA probe on wild-type plants. PNH RNA is undetectable in abaxial cells of cotyledon ([A] to [C]) and leaf primordia (D) (arrows).

(E) to (H) PNH antisense RNA probe on PNH; FIL::PNH transgenic plants. PNH; FIL::PNH plants express PNH mRNA in the wild-type domain and in abaxial cotyledon primordia cells ([E] to [G]) and in abaxial cells near the margin in leaf primordia (H) (arrows).

(I) to (L) GUS antisense probe on FIL::GUS transgenic plants. The activity of the FIL 5′ regulatory sequences used in this analysis is limited to the abaxial cotyledon cells in the embryo ([I] to [K]) and to the abaxial cells in the leaf primordia (L) (arrows).

Bars = 50 μm.