Figure 8.

Gene Expression in pnh; FIL::PNH Embryos.

(A) and (B) In situ hybridization using a CUC2 antisense probe on pnh; FIL::PNH embryos expressing CUC2 in the normal domain (A) or ectopically in the cotyledon primordium (B). Cotyledon stain was visible in multiple sections.

(C) and (D) In situ hybridization using an antisense STM probe on pnh; FIL::PNH embryos. STM is expressed in the normal domain early (C) but also is expressed in the cotyledon primordium by the bent-cotyledon stage, when cotyledon doubling is clearly distinguishable (D).

Black arrows indicate the presumptive SAM, and white arrows indicate the presumed location of ectopic meristem formation. Bars = 50 μm.