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. 1999 Jan 5;96(1):226–231. doi: 10.1073/pnas.96.1.226

Figure 3.

Figure 3

Expression of hTRT, c-myc, and hGAPDH genes in U-87MG glioblastomas xenografted into nude mice or cultured in vitro and treated with MZ-5-156. Total RNA was extracted from the cells or the tumors, amplified by RT-PCR, and electrophoresed on 8% polyacrylamide gel. Lanes 1–3, untreated tumors; lanes 4–6, tumors treated in vivo with MZ-5-156; lane 7, U-87MG cells untreated; and lane 8, U-87MG cells treated in vitro with MZ-5-156. Densitometric quantification of the PCR products showed a 54% decrease (P < 0.05) in the mRNA levels of the hTRT gene in the group treated with MZ-5-156 in vivo as compared with those in the control group. Treatment in vitro of U-87MG glioblastomas with MZ-5-156 decreased the levels of hTRT mRNA by 21% as compared with the controls. MZ-5-156 had no significant effect on the mRNA levels of TP1, hTR, and c-myc after in vivo or in vitro treatment.