Table 1.

Effect of the treatment with the GH-RH antagonist MZ-5-156 on the levels of telomerase activity and mRNA levels of hTRT, hTR, TP1, and c-myc genes in U-87MG glioblastomas xenografted into nude mice

Treatment	Telomerase activity	mRNA levels
		hTRT	TP1	hTR	c-myc
Control	7.5  ±  0.3	1.18  ±  0.008	4.5  ±  0.3	1.5  ±  0.1	1.5  ±  0.2
MZ-5-156	5.1  ±  0.8*	0.54  ±  0.08*	3.9  ±  0.3	1.5  ±  0.1	1.2  ±  0.3
% decrease	32	54	12	0	18

Telomerase activity was assessed by the TRAP assay and expressed as the ratio of the total telomerase product versus the internal control telomerase substrate–nontelomerase. mRNA levels of hTRT, hTR, TP1 and c-myc were assessed by RT-PCR and normalized versus hGAPDH. All values are expressed in arbitrary units. The % decrease in the treated group vs. the controls also is indicated. The results were obtained by using three tumors. 

*P < 0.05 vs. control.