Figure 2.

Effects of Internal Deletions in the N-Terminal Region of TRAB1 on the Ability to Mediate ABA-Responsive Transcription.

Ten micrograms each of the effector plasmid for wild-type (Wt) GBD-TRAB1 or its derivatives with an internal deletion of 20 amino acids (ID1 to ID4) as indicated in the sequence at top and the UAS-TATA-GUS reporter gene containing GAL4 binding sites was cotransfected into protoplasts of rice suspension-cultured cells by electroporation. Each transfection also included 5 μg of a ubiquitin promoter::luciferase (LUC) plasmid as an internal standard. GUS activities were normalized with luciferase activity. The deleted region is indicated by the shaded area in the scheme of each mutant construct. The value of induction by ABA is shown with the ABA + bars. BZ, bZIP.