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ABA-Dependent Phosphorylation of TRAB1-dHA/His Is Abolished by the S102A Mutation.
Protoplast cells transfected with the expression plasmid for wild-type TRAB1-dHA/His (Wt), its S102A mutant derivative (S102A), or the empty vector 35S-shΔ-stop (−) were treated without (−) or with (+) ABA for 1 h. Total cellular extracts were analyzed by immunoblotting with anti-HA antibody. The positions of the S, M, and F bands (see text) are indicated.
