Effects of Mutations at Ser-102 on the Ability of TRAB1 to Mediate ABA-Responsive Transcription.
Cotransfection assays were performed as described for Figure 2 using wild-type (Wt) GBD-TRAB1 or its derivatives that carry mutations at Ser-102. “None” indicates assays using the empty effector plasmid 35S-shΔ-stop. The mutations are illustrated in the sequence at top. The value of induction by ABA is shown with the ABA + bars.
(A) Effect of the S102A mutation.
(B) Effect of the S102D mutation.