Figure 8.

TRAB1 Is Present in the Nucleus Independently of ABA.

(A) Rice suspension-cultured cells (Oc cells) were treated with 50 μM ABA for the indicated times (0 indicates untreated cells). Total cell (T), crude nuclear (N), and postnuclear (PN) fractions were prepared and subjected to immunoblot analysis with anti-TRAB1 antibody. Each lane was loaded with protein corresponding to an approximately equal volume of cells.

(B) Phase contrast and epifluorescence microscopy images deriving from GFP or 4′,6-diamidino-2-phenylindole (DAPI) of a rice protoplast transiently expressing GFP or TRAB1-GFP. Oc cell protoplasts were transformed with a GFP or TRAB1-GFP expression plasmid by electroporation and cultured for 12 h before microscopic observation. Protoplasts were not treated with ABA. DIC, differential interference contrast.