Figure 3.

RNA Gel Blot Analyses of AtEXP7 and AtEXP18 Transcripts in Different Mutant Backgrounds and under Treatment with Ethylene Precursor and Inhibitor.

Total RNA was prepared from roots of 4-day-old wild-type and mutant seedlings. For ACC (5 μM) and AVG (5 μM) treatments, the seedlings were transferred to chemical-containing plates on day 3. Ten micrograms of total RNA, except for Wassilewskija and rhd6 (30 μg), was analyzed. The transcript level of Arabidopsis actin2 (AtACT2) served as a loading control. Col, Columbia wild type; WS, Wassilewskija wild type.