Figure 4.

Effect of 1-MCP on ACC-Induced Root Hair Formation and Expansin Gene Expression in the rhd6 Root.

(A) and (B) Bright-field microscopy images of roots grown in 5 μM ACC without (A) or with (B) 1 μL/L 1-MCP.

(C) and (D) Relative expression levels of AtEXP7 (C) and AtEXP18 (D) in the root when induced by 5 μM ACC without (−MCP) or with (+MCP) 1-MCP. Relative expression levels were evaluated from GFP expression (fluorescence) driven by the AtEXP7 promoter or the AtEXP18 promoter. Bars indicate standard errors (n = 11 to 18).

(E) to (H) Confocal microscopy images of the roots harboring AtEXP7 promoter::GFP ([E] and [F]) and AtEXP18 promoter::GFP ([G] and [H]). Seedlings were incubated in 5 μM ACC without ([E] and [G]) or with ([F]) and [H]) 1-MCP.