Figure 8.

Effect of Translation Inhibitors on Plastid Transcript Levels in hcf109.

Three-week-old mutant and wild-type (WT) plants were incubated with the antibiotics chloramphenicol (CAP) and lincomycin (LM). At time 0 and after 3, 9, and 27 h of incubation, transcripts were isolated and analyzed by RNA gel blot hybridization. Incubation without antibiotics (−) served as a control. Probes of the TGA-containing genes psbB, psbZ, ndhJ, and psaC were used in the analysis. The filters were rehybridized with probes of the rbcL and psbA genes, which contain TAG and TAA stop codons, respectively. Within 27 h, UGA-containing transcripts accumulated in the mutant to wild-type levels. Sizes are given in kb.