Figure 2.

ELISpot assay to quantitate the cellular immune response in spleen and lymph nodes of JAK3 BMT mice 13 days after influenza challenge. Spleen and lymph nodes were collected from infected mice and pooled for analysis. The cells were cocultured with antigen-presenting cells that were either HKx31-infected (CD4) or NP₃₆₆-pulsed (CD8). Uninfected or nonpulsed samples served as negative controls and the background level of IFN-γ production was less than 38 SFC/10⁶ cells. Numbers of IFN-γ producing cells were determined by ELISpot assay in 96-well plates. The JAK3 BMT (gray bars) and +/+ BMT (white bars) mice showed a similar response for both CD4⁺ and CD8⁺ T cells. However, no significant response was detectable in the JAK3 −/− mice (black bars). Results are expressed as the number of SFCs and represent data from three mice assayed as a pool.