Figure 3.

Influenza-specific CD8⁺ T cells in the lungs of JAK3 BMT mice 13 days after influenza challenge. The BAL population was stained for influenza virus-specific CD8⁺ T cells by using the NPP tetramer (NP₃₆₆ + H-2D^b)-phycoerythrin and CD8- fluorescein isothiocyanate, followed by flow cytometric analysis. Significant populations of CD8⁺NPP⁺ cells were found by flow cytometric analysis of BAL cells from the +/+ BMT and JAK3 BMT mice, demonstrating the presence of infiltrating CD8⁺ T cells specific for influenza virus. No significant numbers could be detected in JAK3 −/− mice.