Figure 7.

Influenza-specific cell-mediated cytotoxicity after in vitro stimulation of memory CD8⁺ T cells. Spleen and lymph nodes were collected and pooled from +/+ BMT (open bars) or JAK3 BMT (filled bars) mice that had been infected 130 days previously with the HKx31 virus. After 5 days of in vitro stimulation in the presence of irradiated stimulator cells that were HKx31-infected or untreated, effectors were harvested and cytotoxic T cell activity measured in ⁵¹Cr release assay against HKx31-infected, NP-pulsed, or untreated MC57G (H-2^b) target cells. This result shows lysis of targets at an effector-to-target cell ratio of 3.8:1. The background level of specific lysis was <2% for lymphocytes cultured with uninfected stimulator cells.