Figure 2.

Comparison of Genome Expression Profiles among the Dominant-Negative COP1 Mutant Form (N282), the cop1-4 Mutation, and White Light.

(A) Morphological comparison of continuous white light–grown wild-type, dark-grown N282, cop1-4, and wild-type Arabidopsis seedlings. All seedlings were 6 days old and photographed at the same magnification. D, darkness; N282, seedling overexpressing the N-terminal 282 amino acids of COP1; WL, white light; WT, wild-type seedlings. Bar = 1 mm.

(B) Hierarchical clustering display of expression ratios from wild-type seedlings grown under white light versus darkness, dark-grown N282 seedlings versus dark-grown wild-type seedlings, and dark-grown cop1-4 seedlings versus dark-grown wild-type seedlings. Lane 1, expression ratios of white light– and dark-grown wild-type seedlings; lane 2, expression ratios of dark-grown N282 and wild-type seedlings; lane 3, expression ratios of dark-grown cop1-4 and wild-type seedlings. Only those genes that exhibited twofold or greater differential change in at least one sample pair among the three pairs examined were included for comparison. A total of 2021 genes were included in the cluster (see supplementary data at http://plantgenomics.biology.yale.edu/ for details). The color scale for (B) is shown at bottom right.