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. 2002 Oct;14(10):2383–2398. doi: 10.1105/tpc.004416

Figure 3.

Figure 3.

Kinetics of the Developmental Characteristics and Genome Expression Profile Changes during an in Planta Modulation of COP1 Activity.

(A) Phenotypic characteristics of 6-day-old dark-grown wild-type and cop1-6 mutant seedlings grown at 30°C for different times before transfer to 22°C in the dark. In each panel, the cop1-6 mutant seedlings are shown at left and wild-type seedlings are shown at right. Both the cop1-6 and wild-type seedlings were grown for 0, 0.5, 1, 1.5, 2, 3, 4, 5, and 6 days at 30°C before seedlings were transferred to 22°C. All seedlings were 6 days old and photographed at the same magnification. Bar = 1 mm.

(B) Overview of the hierarchical cluster display of the genome expression profiles among all treated cop1-6 and wild-type sample pairs shown in (A). Each lane represents the expression ratios of 6-day-old dark-grown cop1-6 and wild-type seedlings that were grown initially at 30°C for various times (days are listed at top). A total of 1484 genes that had twofold or greater differential expression in at least one sample pair were included in the cluster (see supplementary data at http://plantgenomics.biology.yale.edu/ for details). The number of genes exhibiting twofold or greater differential expression between dark-grown cop1-6 and wild-type seedlings changed according to their duration of growth at 30°C before being shifted to 22°C; these values are indicated at bottom.

(C) to (E) Three representative subclusters of genes exhibited delayed alteration in their genome expression profiles.

(F) Expression profiles of eight representative genes from some of the cellular and metabolic pathways upregulated in the dark-grown cop1-6 mutant.

(G) Expression profiles of eight representative genes from some of the cellular and metabolic pathways downregulated in the dark-grown cop1-6 mutant.

The expression ratios of genes between the 6-day-old dark-grown cop1-6 and wild-type seedlings shown in (B) to (G) were from seedlings grown at 30°C for 0, 0.5, 1, 1.5, 2, 3, 4, 5, and 6 days before being transferred to 22°C and/or analyzed. The color scale for (B) to (E) is shown at the bottom of (A).