Figure 11.

Subcellular Localization of AtRAC7 and AtRAC8 in Plant Cells in the Absence of FTase or a Functional CaaX Box.

Neither FTase nor a functional CaaX box was required for the association of AtRAC7 and AtRAC8 with the plasma membrane.

(A) GFPAtRAC7 and GFPAtRAC8 were associated with the membranes when expressed transiently in leaf epidermal cells of either wild-type (W.T.) or era1-2 mutant Arabidopsis in which the β-subunit of FTase had been deleted.

(B) GFPAtrac7mCaa− (Atrac7mCaa−) and GFPAtrac8mCaa− (Atrac8mCaa−), in which the last amino acid was deleted, were associated with the plasma membrane after transient expression in N. benthamiana leaf epidermal cells.

Images were acquired using a wide-field fluorescence microscope (A) or a confocal laser scanning microscope (B). Images in (B) are from projection stacks of multiple confocal scans. Bars = 20 μm.